Incucyte® Annexin V Green Dye for Apoptosis
One vial of lyophilized solid sufficient for 100-200 live-cell apoptosis tests in 96-well format.
The Incucyte® Annexin V Green Dye is a highly-selective phosphatidylserine (PS) cyanine fluorescent dye that is specially formulated and ideally suited for real-time measurements of apoptosis in living cultures. The Annexin V Green Dye is validated for use with the Incucyte® Live-Cell Analysis System and employs simple mix-and-read protocols. Addition of the Incucyte Annexin V Green Dye to normal healthy cells is non-perturbing to cell growth and morphology and yields little or no intrinsic fluorescent signal. Once cells become apoptotic plasma membrane PS asymmetry is lost. PS exposure on the extracellular surface enables binding of the Incucyte Annexin V Green Dye resulting in a bright and photostable fluorescent signal. With the Incucyte® integrated analysis software fluorescent apoptotic cells can be quantified and background fluorescence minimized.
The Incucyte Annexin V Green Dye enables real-time evaluation of PS exposure in response to pharmacological agents and/or genetic and environmental factors. Furthermore, the Incucyte Annexin V Green Dye can be combined with the Incucyte® confluence metric, Incucyte® Caspase 3/7 Dye, Incucyte® Nuclight Reagents for nuclear labeling or Incucyte® Cytotox Dyes for multiplexed measurements of apoptosis, cell proliferation or cytotoxicity in every assay well.