The Incucyte® Caspase-3/7 for Metabolism Reagent couples the activated caspase-3/7 recognition motif (DEVD) with a DNA intercalating dye to enable quantification of apoptosis over time. The Caspase-3/7 assay reagent is specially formulated for use in the Incucyte® Live-Cell Analysis System, configured with the SX5 Metabolism Optical Module, and can be added directly to tissue culture wells using a no wash, mix and read protocol to acquire live cell images of cells undergoing caspase-3/7 mediated apoptosis. In addition, the Caspase-3/7 assay reagent is non-perturbing to cell growth and morphology. When added to tissue culture medium, this inert, non- fluorescent substrate crosses the cell membrane where it is cleaved by activated caspase-3/7 resulting in the release of the DNA dye and orange fluorescent staining of nuclear DNA. Kinetic activation of caspase-3/7 can be monitored morphologically using live cell imaging, and quantified using the Incucyte® basic analyzer.