IncuCyte® NeuroLight Orange Lentivirus
Two vials (0.45 mL/vial) of Incucyte® Neurolight Orange Lentivirus (synapsin promoter) suitable for transducing a variety of neuronal cell types in a 96-well format
The Incucyte® Neurolight Orange Lentivirus Reagent is a third generation lentiviral-based vector encoded with an orange fluorescent protein (TagRFP) driven off of a synapsin promoter designed to efficiently transduce multiple cell types. Our extensive validation experiments have shown that expression of this orange fluorescent protein does not negatively alter functional cell biology (e.g. morphology, neurite outgrowth, and neurite branching) of neurons in co-culture with astrocytes. In combination, the Incucyte® SX5 Live-Cell Analysis System or Incucyte® S3 for Neuroscience, Incucyte® NeuroTrack Software (Cat. No. 9600-0010), and the NeuroLight Orange Lentivirus provide an integrated solution for kinetically measuring neurite dynamics in vitro.
Enables quantitation of neurite-specific metrics, such as neurite length, branch points and cell body clusters in co-cultures of neurons or neuronal cell lines with astrocytes
- Efficiently infects multiple neuronal cell types, including cell lines, primary and stem cell-derived cultures
- Synapsin-promoter driven expression of TagRFP, an orange fluorescent protein, ensures minimal expression in non-neuronal cell types
- Reagent is non-perturbing to neuronal cell biology
- Each vial contains 0.45 mL of reagent, sufficient for at least 1 x 96-well plate